Journal: Foods

Article Title: Metabolic Engineering Strategy for Bacillus subtilis Producing MK-7

doi: 10.3390/foods14234150

Figure Lengend Snippet: Tat pathway: TatA, TatC, transport folded proteins across the cell membrane; Sec pathway: SecA, SecDF, SecYEG, transport of unfolded proteins, often occurs immediately after translation; FtsY, receptor of SRP (signal recognition particle) pathway; MFS: transport of small molecules through facilitated diffusion or secondary active transport; ABC transporters: participate in the active transport of a variety of small molecule substrates.

Article Snippet: The Sec pathway constitutes a complete membrane transport complex comprising the transmembrane protein SecYEG, the paratransporting protein SecDF, and the motor SecA [ ].

Techniques: Membrane, Diffusion-based Assay

Journal: Communications Biology

Article Title: How SecB maintains clients in a translocation competent state

doi: 10.1038/s42003-025-08821-2

Figure Lengend Snippet: A Refolding of unfolded MBP (U; left slice) in the presence of SecA 2 (2.5 µM; right panel), as in Fig. . B MBP was allowed to refold in the presence of SecB 4 (1.6 µM) for 5 s and form the SecB-bound state ( B ; left slice) before adding SecA 2 (2.5 µM; right panel), as in Fig. . C SEC-MALS analysis of protein complexes. Top: Individual proteins or complexes (as indicated, 15 µM), used as reference. Bottom: Black line: Unfolded MBP (10 µM) was mixed with SecA 2 (15 µM). Red line: Unfolded MBP (10 µM) and SecB 4 (15 µM) were mixed for 5 s, and then SecA 2 (15 µM) was added. Grey line: Similar to red line, but in a buffer that contained EDTA excess (2 mM). D SDS-PAGE analysis of the indicated fractions from SEC-MALS analysis presented in ( C ).

Article Snippet: Given that only a handful of secretory proteins are SecB clients , , alternative models have been proposed; i.e., selectivity to SecB might relate to the ability of the client to fold while tethered to the chaperone or the substrate-chaperone complex might dictate a relay to a partner such as SecA , .

Techniques: SDS Page